Formamide ACS Reagent ≥99.5% 100 ml
Synonym: Amide C1, Formic amide
- CAS Number 75-12-7
- Linear Formula HCONH2
- Molecular Weight 45.04
- Beilstein/REAXYS Number 505995
- EC Number 200-842-0
- MDL number MFCD00007941
- PubChem Substance ID 24853220
| Grade | ACS reagent |
| Vapor Density | 1.55 (vs air) |
| Vapor Pressure | 0.08 mmHg (20°C) 30 mmHg (129°C) |
| InChI Key | ZHNUHDYFZUAESO-UHFFFAOYSA-N |
| Assay | ≥99.5% |
| Autoignition Temperature | 932°F |
| color | APHA: ≤10 |
| Refractive Index | n20/D 1.447 (lit.) |
| bp | 210°C (lit.) |
| mp | 2-3°C (lit.) |
| transition temp | freezing point 2.0-3.0°C |
| Density | 1.134 g/mL at 25°C (lit.) |
Application
The addition of formamide may enhance the specificity and efficiency of amplification of PCR (polymerase chain reaction). It may be used as an ammonia equivalent for the palladium-catalyzed aminocarbonylation of 1-bromo-3-trifluoromethylbenzene to form 3-(trifluoromethyl)benzamide.
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
Safety Information
Signal word Danger
Hazard statements H351-H360D-H373
Precautionary statements P201-P281-P308 + P313
Target organs Blood
Personal Protective Equipment Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
RIDADR NONH for all modes of transport
WGK Germany 1
RTECS LQ0525000
Flash Point(F) 347°F
Flash Point(C) 175°C





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